| Name: Robert M Silvers |
Job Position Title: Research Scientist
Email:
Education Level: M.S.
Objective: I am a motivated scientist searching for a challenging full-time position in the biotechnology/pharmaceutical industry. I am qualified in many areas of research including phage display technology, cell culture, molecular biology and microbiology.
Education: Master of Science, Microbiology, Thomas Jefferson University, Philadelphia, Pa., Aug. 2009
Thesis: “Modification of Integration Site Preferences of an HIV-1-based Vector by Expression of a Novel Synthetic Protein”
Bachelor of Science, Biotechnology, Kent State University, Kent, Ohio, Dec. 2006
Experience Level: 3 to 5 Years
Experience:
Thomas Jefferson University June 2007 to Oct. 2009
Division of Infectious Diseases
Philadelphia, Pa.
Research Technician
Yoh Scientific, assigned at Merck and Co., Inc. Oct. 2009 to present
Department of Biologics
Merck Research Labs, West Point, Pa.
Biochemist II, 12 month contract position
Skills:
Performed restriction-ligation cloning to create gene mutants.
Utilized several different types of PCR including inverse, linker-mediated and Alu
Achieved high quality yields using QIAGEN kits designed for plasmid DNA purification, genomic DNA preparation, and agarose gel extraction.
Carried out other techniques such as gel electrophoresis, TOPO-TA cloning, primer design, restriction digests, ligations, DNA sequencing analysis.
Maintained common laboratory cell lines such as 293T, NIH3T3 and HeLa.
Performed experiments involving transfections, transductions and cytotoxicity assays.
Produced replication-deficient HIV-1-based viral particles in 293T cells using a three-plasmid expression system.
Prepared media and computed basic calculations for cell counting and plating.
Assay development for profiling and validation of phage display antibody libraries.
Performed phage display antibody library selection (library panning) using several libraries and multiple antigens.
Screened for positive clones using 96- and 384- well Fab or phage sandwich ELISA.
Extensive use of Morphosys AutoCAL software and robots for high-throughput ELISA screening.
Sequencing and data analysis of high binding clones.
Presented research at several group and departmental meetings.
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